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The activities of the DNA polymerases of calf thymus in synthesizing primed M13 phage DNA and primed poly(dA).(dT)12-18 by means of an endogenous deoxyribonuclease have been characterized. The patterns of the incorporation of the four nucleoside triphosphates into the primed DNA were somewhat different for the different polymerases. Also, differences were observed in the incorporation of dATP by the DNA polymerase-beta, which may reflect its subunit structure (Alderton, P.B., and Lees, M.J. (1986) J. Biol. Chem. 261, 15901-15905). Finally, a new method to investigate the mode of nucleotide analogue inhibition of DNA polymerases has been developed based on the use of poly(dA).(dT)16-18 as a primed template for DNA synthesis. Incorporation of trifluorothymidine triphosphate in the presence of poly(dA).(dT)16-18 had a pattern similar to that of dTTP, but lagged behind. A kinetic model for the incorporation of nucleotide analogue inhibitors is proposed, and it is shown that this model is consistent with known features of the hydrolytic activity of DNA polymerases. current conclusions.
It will be interesting to see where this debate goes when the evidence becomes more complete.
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A few notes:
The data being compared here are children with severe asthma, who have benefited greatly from inhaled medications – and these are children who have responded to those medications. In fact, in the studies reviewed here, there was no placebo group to evaluate what would have happened without medications. By including the most severe cases, we hope to