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ApocalypseFlipperDLCTorrentdownloadIsolation of oil contamination from soil using molecular biomarkers for membrane hydrocarbons.
Hydrocarbons in soil can be originated either from living or dead organisms; however, the distribution and mechanisms of anthropogenic transport to soil of the two are distinct. This study investigates the application of molecular biomarkers to distinguish between the sources of hydrocarbons in contaminated soil. Biomarkers, including DNA, rRNA and stable isotope ratios (δ(13)C), were extracted and analyzed from a variety of contaminated soils including uncontaminated, oil-contaminated, and non-oil-related arctic soils. We further tested how the amount and quality of the biomarker templates are influenced by DNA extraction methods. The distribution of biomarkers among the contaminated samples was analyzed using principal component analysis. A discrimination model based on biomarker data was constructed and validated, and the applicability of the model to the other samples was tested. The results showed the biomarkers could be used as a potential indicator to distinguish the origin of hydrocarbons in soil.No significant association between the PRAQQ single nucleotide polymorphism rs8887 and cerebral infarction.
Cerebral infarction (CI) is a common cerebrovascular disease. An association has been identified between the PLA(2)R1 rs6595804 single nucleotide polymorphism (SNP) and CI. The aim of this study was to investigate whether the proline-arginine-alanine-alanine-glutamine (PRAQQ) gene is associated with CI. The Chinese population was genotyped for the PRAQQ rs8887 (G3663C) SNP using polymerase chain reaction-restriction fragment length polymorphism. A total of 626 case patients and 638 control subjects were included in the study. When the cases and controls were combined, the PRAQQ rs8887 GG genotype was significantly associated with CI in a dominant model (OR = 1.458, 95% CI = 1.084-1.952, P = 0.0143). When the controls were analyzed, the PRAQQ rs8887 CC genotype was significantly associated with CI in a dominant model (OR = 1.665, 95% CI = 1.124-2.461, P = 0.0119). This association was not observed in the study of the combined cases and controls.
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